Thin Layer Chromatography (TLC) Test Kit

The Thin Layer Chromatography (TLC) Test Kit provides all the necessary tools and materials to perform effective TLC analysis. Thin Layer Chromatography is a simple and cost-effective method to separate and analyze compounds in mixtures, widely used in laboratories for chemical, biological, and food analysis. This kit includes high-quality glassware, tools, and reagents, making it ideal for both educational and professional laboratory environments.

Properties:

• Complete TLC Setup: Includes everything needed to perform Thin Layer Chromatography, from TLC sheets to solvents and indicators.
• Durable Glassware: High-quality glass components like measuring cylinders, beakers, and funnels ensure accurate and reliable performance.
• Precision Tools: Includes stainless steel tweezers, capillary tubes, and spring clips for precise sample handling and analysis.

Specification:

• Screw Top Square Jar: 250ml, for mixing and storing solutions.
• Tweezers (Stainless Steel): 125mm, for delicate handling of TLC sheets and samples.
• Spring Clip (Large): Holds TLC sheets securely in place during analysis.
• Measuring Cylinder (Graduated Glass): 10ml for precise measurement of liquids.
• Filter Funnel (Glass): 50mm for filtering solvents and reagents.
• Squat Beakers (Graduated Glass): 4 x 100ml for mixing and preparing samples.
• Thin Layer Chromatography (TLC) Sheet: 200 x 200mm, Macherey-Nagel brand, for separating and analyzing samples.
• TLC Capillary Tubes: 100 per vial, for precise spotting of samples onto TLC sheets.
• Black Lead Pencil (HB) & Clear Perspex Ruler: 30cm, for accurate marking and measurement of TLC sheets.
• White A4 Paper: For documenting results and calculations.

Reagents:

• TLC Standard Malic Acid: 25ml, 2g/L concentration for standard comparisons.
• TLC Standard Lactic Acid: 25ml, 2g/L concentration for standard comparisons.
• TLC Solvent Mixture: 100ml, used for sample separation during chromatography.
• TLC Indicator: 100ml, used to visualize spots and track progress during analysis.

Uses and Application:

• Chemical Analysis: Ideal for separating and identifying compounds in mixtures, such as organic acids and alkaloids.
• Food Testing: Commonly used in food laboratories to identify and quantify components like malic and lactic acids.
• Research and Education: Perfect for academic labs to teach chromatography techniques or conduct research.

Storage:

• Store reagents and TLC sheets in a cool, dry place away from direct sunlight and heat.
• Glassware should be cleaned thoroughly after use and stored in a secure location to prevent breakage.

Hazardous Information:

• Handle solvents and reagents with care, as they may be flammable or irritant.
• Avoid direct contact with skin and eyes; wear appropriate PPE (gloves, goggles).

Safety Measures:

• Always wear protective gear such as gloves, goggles, and lab coats when handling chemicals and performing chromatography.
• Ensure proper ventilation when using solvents and reagents.
• Follow standard laboratory safety protocols and dispose of chemicals responsibly.